pfuse higg1 fc2 plasmid Search Results


mammalian expression plasmid fusion-higg1-fc2 tag vector (pfuse-higg1-fc2  (GSL Biotech)
90
GSL Biotech mammalian expression plasmid fusion-higg1-fc2 tag vector (pfuse-higg1-fc2
Graphical map of the constructed plasmid vector and PCR analysis of GD2 scFv gene. (A) Graphical map of <t>pFUSE-hIgG1-Fc2</t> vector created by SnapGene software is shown. Nucleotide sequence of mouse scFv against GD2 was cloned into the vector at EcoRI and NcoI sites before the hinge region of the hIgG1 Fc part. The thin orange arrow indicates an open reading frame, which starts at the codon inside IL2ss and stops at the end of the Fc portion. Zeocin drug resistance gene ( Zeo ) was used for selection; (B) after cloning step, the constructed vector was transformed into DH5-α E. coli and selected by zeocin drug. Five bacterial colonies (as indicated) were picked up and grown. PCR was performed to detect the inserted GD2 scFv gene inside the constructed plasmid vectors. Bacteria clone number 1 was chosen for plasmid extraction. Standard DNA markers (M) in base pairs are shown on the left. Positive and negative controls are also demonstrated. Neg: negative; Pos: positive
Mammalian Expression Plasmid Fusion Higg1 Fc2 Tag Vector (Pfuse Higg1 Fc2, supplied by GSL Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
mammalian expression plasmid fusion-higg1-fc2 tag vector (pfuse-higg1-fc2 - by Bioz Stars, 2026-09
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Graphical map of the constructed plasmid vector and PCR analysis of GD2 scFv gene. (A) Graphical map of pFUSE-hIgG1-Fc2 vector created by SnapGene software is shown. Nucleotide sequence of mouse scFv against GD2 was cloned into the vector at EcoRI and NcoI sites before the hinge region of the hIgG1 Fc part. The thin orange arrow indicates an open reading frame, which starts at the codon inside IL2ss and stops at the end of the Fc portion. Zeocin drug resistance gene ( Zeo ) was used for selection; (B) after cloning step, the constructed vector was transformed into DH5-α E. coli and selected by zeocin drug. Five bacterial colonies (as indicated) were picked up and grown. PCR was performed to detect the inserted GD2 scFv gene inside the constructed plasmid vectors. Bacteria clone number 1 was chosen for plasmid extraction. Standard DNA markers (M) in base pairs are shown on the left. Positive and negative controls are also demonstrated. Neg: negative; Pos: positive

Journal: Exploration of Targeted Anti-tumor Therapy

Article Title: Chimeric single-chain variable fragment-human immunoglobulin G crystallizable fragment antibody against GD2 for neuroblastoma targeted immunotherapy

doi: 10.37349/etat.2023.00188

Figure Lengend Snippet: Graphical map of the constructed plasmid vector and PCR analysis of GD2 scFv gene. (A) Graphical map of pFUSE-hIgG1-Fc2 vector created by SnapGene software is shown. Nucleotide sequence of mouse scFv against GD2 was cloned into the vector at EcoRI and NcoI sites before the hinge region of the hIgG1 Fc part. The thin orange arrow indicates an open reading frame, which starts at the codon inside IL2ss and stops at the end of the Fc portion. Zeocin drug resistance gene ( Zeo ) was used for selection; (B) after cloning step, the constructed vector was transformed into DH5-α E. coli and selected by zeocin drug. Five bacterial colonies (as indicated) were picked up and grown. PCR was performed to detect the inserted GD2 scFv gene inside the constructed plasmid vectors. Bacteria clone number 1 was chosen for plasmid extraction. Standard DNA markers (M) in base pairs are shown on the left. Positive and negative controls are also demonstrated. Neg: negative; Pos: positive

Article Snippet: Nucleotide sequence of mouse scFv against GD2 clone 14G2a (a kind gift from Prof. Dr. Suradej Hongeng, Department of Pediatrics, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand) was designed to be inserted into mammalian expression plasmid fusion-hIgG1-Fc2 tag vector (pFUSE-hIgG1-Fc2) using SnapGene software (version 1.1.3, GSL Biotech LLC, San Diego, CA, USA).

Techniques: Construct, Plasmid Preparation, Software, Sequencing, Clone Assay, Selection, Transformation Assay, Bacteria, Extraction

Determination of scFv-IgG Fc expression in transfected HEK293T cells. (A) Human HEK293T cell line was transfected with pFUSE-scFv-GD2-hIgG1-Fc2 vector and intracellular stained with Alexa Fluor 488-conjugated goat anti-hIgG antibody (GαhIgG-A488), Alexa Fluor 488-conjugated goat anti-mouse IgG antibody (GαmIgG-A488), or without antibodies (no Abs); (B) transfected HEK293T cells were cultured in the presence of zeocin. The transfected cells and un-transfected cells were intracellularly stained with Alexa Fluor 488-conjugated goat anti-hIgG antibody (Blue color) or Alexa Fluor 488-conjugated goat anti-mouse IgG antibody (red color). Overlay histograms are shown

Journal: Exploration of Targeted Anti-tumor Therapy

Article Title: Chimeric single-chain variable fragment-human immunoglobulin G crystallizable fragment antibody against GD2 for neuroblastoma targeted immunotherapy

doi: 10.37349/etat.2023.00188

Figure Lengend Snippet: Determination of scFv-IgG Fc expression in transfected HEK293T cells. (A) Human HEK293T cell line was transfected with pFUSE-scFv-GD2-hIgG1-Fc2 vector and intracellular stained with Alexa Fluor 488-conjugated goat anti-hIgG antibody (GαhIgG-A488), Alexa Fluor 488-conjugated goat anti-mouse IgG antibody (GαmIgG-A488), or without antibodies (no Abs); (B) transfected HEK293T cells were cultured in the presence of zeocin. The transfected cells and un-transfected cells were intracellularly stained with Alexa Fluor 488-conjugated goat anti-hIgG antibody (Blue color) or Alexa Fluor 488-conjugated goat anti-mouse IgG antibody (red color). Overlay histograms are shown

Article Snippet: Nucleotide sequence of mouse scFv against GD2 clone 14G2a (a kind gift from Prof. Dr. Suradej Hongeng, Department of Pediatrics, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand) was designed to be inserted into mammalian expression plasmid fusion-hIgG1-Fc2 tag vector (pFUSE-hIgG1-Fc2) using SnapGene software (version 1.1.3, GSL Biotech LLC, San Diego, CA, USA).

Techniques: Expressing, Transfection, Plasmid Preparation, Staining, Cell Culture